Marble Burying Test Autism

The marble burying test protocol described below is modified from deacon 2006 and angoa perez kane.

Marble burying test autism.

Marble burying is an animal model used in scientific research to depict anxiety or obsessive compulsive disorder ocd behavior. Each mouse was placed for 45 min into a clean rat cage with 4 cm deep bedding and 12 glass marbles placed on the surface. The marble burying test is a useful model of both obsessive compulsive like ocd behavior and or anxiety like behavior. Santini et al 2013.

When put in a cage with marbles mice with ocd like symptoms tend to engage in a high degree of repetitive behaviors including digging while mice with a high degree of anxiety tend to engage in a high degree of digging in novel contexts potentially as a means to attempt. For the communication domain neonatal ultrasonic vocalization and olfactory habituation test toward social and non social odor are described. It is based on the observation that rats and mice will bury either harmful or harmless objects in their bedding. While widely used there is significant controversy over the interpretation of its results.

The number of marbles that were buried by the mouse was measured. Zimeldine 10 0 mg kg reduced marble burying without affecting locomotor activity. Table 3 marble burying behavior in a two compartment box no. Springer nature is developing a new tool to find and evaluate protocols.

In the current study significant differences in marble burying behavior were observed between pm 2 5 exposed and control rats figure 1b and supplementary figure 3 f 3 35 37 56 p 001. The marble burying test takes advantage of the proclivity of mice to dig in natural settings e g. The marble burying test is commonly used to detect repetitive digging behavior servadio et al 2015. Behavior obsessive compulsive disorder autism spectrum disorder mouse marble burying this is a preview of subscription content log in to check access.

In this unit behavioral tests are described that are relevant to the domains affected in asd. Of duration of marbles buried test s mean sem time of nonmarble side s mean sem time nonmarble side 300 600 1800 4 2 2 0 7 3 0 9 8 4 0 4 143 4 292 30 916 45 47 ns 49 ns 51 ns data from 10 female. Burrows escape tunnels and in standard cage bedding and the nestlet shred test capitalizes on the fact that mice are nesting animals by nature that build nests for protection of themselves and their offspring against environmental conditions e g. The marble burying test in animal models of asd such as the btbr t tf j mouse eif4e transgenic mice 4e tg as well as in the maternal immune activation mouse model amodeo et al 2012.

For the repetitive domain the t maze spontaneous alternation test and marble burying test are described. Marble burying test and self grooming behavior.